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(A) Titration of 461A into TcFPPS (a) fits a two-sequential binding site model (c) better than a one-site model (b). (B) Isotherms of ITC experiments by titrating 91B, 261A, 300B, 476A, or risedronate into TcFPPS all fit a two-sequential binding site model. The sharp slope at the very beginning of the isotherm (blue arrows) is only predicted by a two-sequential site but not a two-permanent site binding model.
