Table 1. Quantitative Changes of Phagosomal Proteins Regulated by ManLAM Treatmenta.
| biological process | protein name | ManLAM | PILAM | LPSb |
|---|---|---|---|---|
| Down-Regulated Proteins | ||||
| Membrane receptor/Signaling | Mannose-6-phosphate receptor | 0.75 | 1.04 | 1.30 |
| CD63 | 0.67 | 1.06 | 1.27* | |
| Scavenger receptor class B member 2 | 0.66 | 1.05 | 1.23* | |
| Membrane structure and lipid rafts | Lysosomal membrane glycoprotein 1 | 0.60 | 0.94 | 1.08* |
| Lysosomal membrane glycoprotein 2 | 0.76 | 1.02 | 1.02 | |
| Transmembrane protein 55A | 0.79 | 0.80 | 0.89 | |
| Vesicular and protein trafficking | Early endosome antigen 1 | 0.55 | 0.96 | 1.04 |
| Vesicle transport through interaction with t-SNAREs homologue 1B | 0.75 | 1.03 | 1.28 | |
| Rab-7A | 0.71 | 1.08 | 1.14 | |
| Rab-7B | 0.65 | 0.86 | 1.12* | |
| Molecular transport | Immune regulator 1, ATPase, H+ transporting | 0.72 | 0.99 | 1.31* |
| ATPase, H+ transporting, lysosomal V0 subunit a | 0.75 | 0.95 | 1.04* | |
| Vacuolar ATP synthase subunit B | 0.74 | 1.04 | 1.42 | |
| Vacuolar ATP synthase subunit E1 | 0.65 | 0.92 | 1.35 | |
| Vacuolar ATP synthase subunit S1 | 0.69 | 0.89 | 0.99 | |
| Vacuolar ATP synthase subunit C | 0.79 | 1.09 | 1.40 | |
| Vacuolar ATP synthase subunit F | 0.79 | 1.25 | 1.34 | |
| Vacuolar ATP synthase subunit d | 0.71 | 0.98 | 1.15* | |
| Vacuolar ATP synthase catalytic subunit A | 0.79 | 0.96 | 1.28 | |
| Isoform 1 of Reticulon-4 | 0.78 | 0.88 | 0.94 | |
| Hydrolases | Cathepsin Z | 0.74 | 0.97 | 0.98 |
| Lysosomal acid phosphatase | 0.75 | 1.15 | 1.23 | |
| Metabolism | Collectin subfamily member 12 | 0.79 | 0.92 | 0.88 |
| Uncharacterized | Zinc finger, FYVE domain containing 26 | 0.60 | 0.93 | 1.27 |
| UP-Regualted Proteins | ||||
| Membrane receptor/Signaling | Transferrin receptor protein 1 | 1.34 | 0.96 | 1.05 |
| Vesicular and protein trafficking | Vacuolar protein sorting-associated protein 41 homologue | 1.50 | 0.96 | 1.04* |
| Rab-5A | 1.33 | 1.10 | 0.91 | |
| Rab-5C | 1.29 | 0.96 | 0.98 | |
| Rab-14 | 1.67 | 1.17 | 1.13* | |
| Signaling | Isoform 1 of Sequestosome-1 | 1.39 | 1.06 | 1.12 |
| Isoform 2 of Sequestosome-1 | 1.82 | 1.10 | 1.19 | |
| Iron homeostasis | Ferritin H subunit | 1.50 | 1.28 | 1.06* |
| Protein folding | Calnexin | 1.53 | 1.01 | 1.40 |
| Protein folding/antigen presentation | Heat-shock protein 90B1 | 1.48 | 0.77 | 1.31 |
| Hydrolases | Tripeptidyl-peptidase 1 | 1.32 | 1.21 | 1.08 |
| Metabolism | N-acetylglucosamine-6-sulfatase | 1.26 | 1.09 | 1.20 |
| d-3-phosphoglycerate dehydrogenase | 1.58 | 1.10 | 1.20* | |
| Alpha-N-acetylglucosaminidase | 1.37 | 1.08 | 1.07 | |
| Cell mobility and cytoskeleton | Vimentin | 1.82 | 1.12 | 1.26 |
| Titin | 1.28 | 0.99 | 1.20 | |
| Uncharacterized | 24 kDa protein | 1.30 | 0.99 | 1.02 |
| Ddost | 1.31 | 0.98 | 1.18 | |
a
The protein ratios represent the relative abundance of a specific protein in the phagosome bearing latex beads coated with ManLAM, PILAM, or LPS vs uncoated beads. Macrophage cells were treated by latex beads coated with three different bacterial lipoglycans or uncoated beads, and proteins were extracted from phagosomal membranes for quantitative comparison using iTRAQ isotopic labels. The protein ratios indicate relative changes of protein levels in the phagosomes under a specific lipoglycan treatment in comparison with no treatment. Forty-two proteins were found to be significantly changed only by ManLAM treatment, which were summarized in this table.
b
The ratio with an asterisk is the mean of two replicate measurements.