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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Nat Cell Biol. 2010 Dec 19;13(1):49–58. doi: 10.1038/ncb2133

Figure 2. DDR1 does not require kinase activity or collagen binding to regulate acto-myosin at cell contacts.

Figure 2

(a) Representative pictures where DDR1 resistant to siRNA oligonucletides #3&#4 was expressed in two DDR1 stable knock down clones (shDDR1#3 and shDDR1#4) or control cells (shCtr). F-actin is shown in red and DDR1 in green. The bottom panels show shDDR1#3 cells transfected with cherry as a control; scale bars are 10 μm. (b) The upper graph shows the quantification of recovery of normal actin organisation in two DDR1 stable knock down clones (shDDR1#3 and shDDR1#4) or control cells (shCtr) transfected with cherry as a control or with a DDR1 construct resistant to siRNA oligonucleotides #3&#4. Left panels show when DDR1 (or cherry) is expressed only in one of the two cells in contact, while right panels show when DDR1 (or cherry) is expressed in both two cells in contact with one another. In the bottom graph is represented the percentage of cells with normal actin organisation in the cell-cell contacts in shDDR1#3 and shDDR1#4 cells transfected with cherry as a control or with different DDR1 constructs resistant to siRNA oligonucleotides #3&#4 (wt, K618A, R105A or ΔDS1). Average of three experiments is shown: 20-30 cells were counted per experiment. (c) F-actin (left) and pS19-MLC organisation in A431 cells transfected with ECTM-GFP (wt in top panels and R105A in bottom panels). In the top panels, cells on the left are non-transfected cells. Scale bars are 20 μm. (d) example of DDR1 staining (green) in apical (top panel) and basal (bottom panel) membranes of A431 cells. Scale bars are 20 μm. (e) F-actin and DDR1 staining is shown in A431 cells transfected with control or E-cadherin siRNA. Scale bar is 20μm. (f) Western blot showing E-cadherin and DDR1 levels in A431 cells transfected with control or E-cadherin siRNA.