Figure 12.

Assessment of Ag2/PRA-specific response in DC-vaccinated mice. The antigen-recall response was assessed by ELISPOT assay in lymphocytes harvested from lung, thymus and lymph nodes of DC-vaccinated mice. The numbers of IFN-γ, IL-4 and IL-17A-secreting lymphocytes were counted by ELISPOT assay. The spots obtained were normalized per one million cells and subtracted those from control (mice injected with DCs transfected with vector plasmid DNA). The results shown here are mean (SEM) values obtained from 5 mice in each group.