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. 2011 Jan 10;6(1):e15759. doi: 10.1371/journal.pone.0015759

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Ahmad et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative examples of fluorescent photomicrographs of Ang-(1–12) immunoreactivity of the cultured myocytes (maintained for 48 hours in serum-deprived medium) from WKY (A and C; top panel) and SHR (B and D; bottom panel) with protein A purified Ang-(1–12) polyclonal antibody (1∶100 dilution). (A and B) represents the fluorescent staining of endogenous Ang-(1–12) with antibody while (C and D) illustrates the blocking of fluorescent staining of cardiac myocytes after preadsorption of the antibody with 10 µM of synthetic rat Ang-(1–12) peptide. Magnification, X400.