Table 1. FACS profile for cell cycle analysis with ovarian cancer cells that were grown under cell culture conditions consisting of increasing serum and O2 concentration.
Cells | Serum | G1 | S | G2 | |||||||
3% O2 | 21% O2 | p-value | 3% O2 | 21% O2 | p-value | 3% O2 | 21% O2 | p-value | |||
2% | 66.8±0.9 | 62.5±0.2 | 0.0011 | 25.6±1 | 27.5±0.1 | 0.02800 | 7.6±0.4 | 9.96±0.1 | 0.0007 | ||
A2780 | 6% | 76.9±2.1 | 64.3±0.7 | 0.0006 | 17.5±0.9 | 29±0.1 | <0.0001 | 5.6±1.4 | 6.7±0.6 | ** NS ** | |
10% | 84.1±3.2 | 69.5±1.4 | 0.0028 | 14.3±1.9 | 26.5±1.3 | 0.0080 | 1.5±1.3 | 4±0.7 | 0.0411 | ||
2% | 66.4±0.7 | 60.2±0.4 | 0.0001 | 25.8±0.2 | 29.3±0.6 | 0.0006 | 7.7±0.5 | 10.4±0.3 | 0.0017 | ||
OVCAR5 | 6% | 79±1.5 | 70.2±0.5 | 0.0014 | 17.4±1.5 | 25.1±0.9 | 0.0015 | 3.4±0.4 | 4.7±0.6 | ** NS ** | |
10% | 90.6±1.2 | 84±1.6 | 0.0077 | 8.2±1.4 | 14.6±1.5 | 0.0052 | 1.1±0.1 | 1.2±2.1 | ** NS ** | ||
2% | 74.7±10.7 | 69±1 | ** NS ** | 15.5±2.5 | 23.7±0.6 | 0.0052 | 13.7±2.3 | 6±0 | 0.0043 | ||
OVCAR8 | 6% | 66±1 | 59±1 | 0.0010 | 25.7±1.5 | 30±0 | 0.0079 | 7±0 | 9.7±0.6 | 0.0013 | |
10% | 69±4.6 | 61.7±1.5 | 0.0582 | 24.3±1.5 | 30.7±1.2 | 0.0045 | 5.3±3.8 | 6.3±0.6 | ** NS ** | ||
2% | 69.3±1.5 | 58.7±2.1 | 0.0020 | 25±1 | 34±1 | 0.0003 | 4.3±1.5 | 5.7±1.5 | ** NS ** | ||
HeyA8 | 6% | 62.7±0.6 | 53.7±1.5 | 0.0006 | 30.3±1.2 | 37.3±2.5 | 0.0118 | 5.3±0.6 | 8±3.5 | ** NS ** | |
10% | 61.7±1.2 | 50.3±2.3 | 0.0016 | 30.7±1.2 | 37.3±1.2 | 0.0021 | 6.7±2.3 | 11±1.6 | ** NS ** | ||
2% | 80±1.7 | 72.3±1.5 | 0.0045 | 15±0 | 18.7±1.5 | 0.0141 | 5±1.7 | 8±1 | ** NS ** | ||
HOC8 | 6% | 80.3±1.2 | 72.7±1.2 | 0.0012 | 15.3±1.5 | 22.7±1.5 | 0.0041 | 3.3±1.5 | 3.7±1.5 | ** NS ** | |
10% | 78.7±2.5 | 73.3±0.6 | 0.0232 | 16.3±0.6 | 23.7±0.6 | <0.0001 | 4.3±2.5 | 3±1 | ** NS ** | ||
2% | 74±2 | 62.1±1.7 | 0.0014 | 13.5±3.5 | 27.7±1.2 | 0.0026 | 12±2 | 10.3±2.5 | ** NS ** | ||
SKOV3 | 6% | 76.7±1.5 | 65.3±3.2 | 0.0052 | 14±1 | 25.7±1.5 | 0.0003 | 9.3±1.5 | 9±2 | ** NS ** | |
10% | 80.3±1.2 | 68±1.7 | 0.0005 | 12.7±1.5 | 26±1 | 0.0002 | 7±1 | 6.3±0.6 | ** NS ** |
**NS** “Not Significant”