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. 2010 Dec 16;9:90. doi: 10.1186/1475-2840-9-90

Figure 3.

Figure 3

Diabetic CAECs produce and release greater amounts of IL-6 and IL-8 in response to TLR2 and TLR4 stimulation. Non-diabetic and diabetic CAECs were stimulated with PGN (10 μg/ml) and LPS (200 ng/ml) for 1, 2 or 24 h. Levels of IL-6 and IL-8 peptides (A and B) in medium were assessed by ELISA after treatment for 24 h, and mRNA levels in cell lysates (C, D, E and F) were analyzed with real-time PCR after treatment for 1 or 2 h. After stimulation of either TLR2 or TLR4, diabetic cells expressed higher levels of IL-6 and IL-8 mRNA and released greater amounts of IL-6 and IL-8. Results are expressed as Mean ± SEM; n = 5; *P < 0.05 vs. control; #P < 0.05 vs. non-diabetic cells treated with LPS or PGN.