Table 4. Elimination of btuE results in increased thiobarbituric acid-reactive substances in E. coli.
pmol TBARS/mg protein | |||
Strain | Control | K2TeO3 | H2O2 |
BW25113 | 24.6±5.7 | 81.20±8.0 | 131.9±18.6 |
ΔbtuE | 162.0±9.0 | 147.0±27.1 | 144.0±2.5 |
Membrane lipid peroxidation products were determined as thiobarbituric acid-reactive substances (TBARS) in wild type (BW25113) and ΔbtuE strains in the absence (control) or presence of K2TeO3 (0.5 µg ml−1) or H2O2 (100 µM) for 30 min. Values represent the mean of three independent experiments ± SD.