Table 1.

Survival analysis for 50 GBM patients treated with STUPP protocol.

Variable	Univariate Cox Regression			Log Rank Test		Multivariate Cox Regression
	HR	95% CI	p-value	ß cut-off	p-value	HR	95% CI	p-value
Age (≥ 50 yr vs. < 50 yr)	1.8	0.9 - 3.9	0.1	-	-	-	-	-
Sex (male vs. female)	1.5	0.8 - 2.8	0.3	-	-	-	-	-
KPS (≥ 80 vs. < 80)	1.2	0.6 - 2.3	0.6	-	-	-	-	-
TBX3	113	15.1 - 851.9	4.0E-06	0.45	1.0E-08	0.05	0.01 - 0.2	3.0E-05 (*)
FSD1	18	2.9 - 112	0.002	0.70	3.0E-07	0.2	0.09 - 0.6	0.002 (*)
FNDC3B	0.08	0.01- 0.4	0.002	0.55	7.0E-05	3.1	1.4 - 6.9	0.005
DGKI	77	9.5 - 616.5	4.0E-05	0.45	3.0E-06	0.3	0.1 - 0.7	0.008
FLJ25422	0.05	0.007 - 0.3	0.002	0.70	4.0E-04	2.9	1.2 - 7.2	0.02
SEPP1	0.008	0.003 - 0.2	0.004	0.10	2.0E-04	2.3	1.1 - 4.7	0.02
SOX10 #1	10	1.6 - 67.2	0.01	0.70	1.0E-04	0.4	0.2 - 0.9	0.03
CCND1	31	4.3 - 216	6.0E-04	0.75	2.0E-04	2.3	0.9 - 5.5	0.1
SOX10 #2	12	1.9 - 74.8	0.008	0.80	4.0E-04	0.5	0.2 - 1.2	0.1
ZNFN1A3	0.11	0.02 - 63.8	0.02	0.35	9.0E-04	1.8	0.8 - 4.2	0.2
MGMT	0,18	0.04 - 0.8	0.02	0.10	9.0E-06	-	-	-

Log rank tests were performed between methylated and non-methylated patients. The multivariate analysis includes the methylation status of the tested CpG site and MGMT. MGMT methylation status was always significantly associated with OS (p-value < 0.05) and (*) indicates which CpG site had a lower p-value than the one observed for MGMT in the multivariate model. (yr = year; HR = Hazard Ratio; CI: Confidence Interval).