Fig. 6.
Ca2+ chelation within nuclei or extra-nuclear cytoplasm arrests HSC at the G2 phase of the cell cycle. LX-2 cells were either untransfected or transfected with DsRed, PV-NLS-DsRed, or PV-NES-DsRed, and cell cycle was determined by FACS analysis. Relative to control or DsRed-transfected cells, the fraction of cells in G2/M phase was increased from 33–35% to 46–48%, with a consequent decrease in cell fraction in S phase from 30–33% to 18–23%. No change in fraction of cells in G1 phase was noted, as the fraction in all cells was 31–35%.