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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Antiviral Res. 2010 Nov 12;89(1):54–63. doi: 10.1016/j.antiviral.2010.11.004

Figure 5. Effect of IFN-α and a 2'-C-methyladenosine nucleoside analogue NS5B polymerase inhibitor on JFH-ΔV3-Rluc reporter virus replication in cell culture.

Figure 5

Panel A, two hours post-infection with the JFH-ΔV3-Rluc reporter virus (moi=0.1) cells had increasing concentrations of IFN-α added followed by two days of incubation (see Materials and Methods). The levels of Renilla luciferase in cell lysates were determined as described in Figures 2–4. Cell cultures were performed in triplicate in 96-well plates, the experiment was done twice and results are reported in relative light units (RLU) as mean ± standard deviation (n=6). The figure shows a representative result of three independent experiments. Panel B, As an additional control, the effect of a well studied 2'-C-methyladenosine nucleoside analogue NS5B polymerase inhibitor was tested in the JFH-ΔV3-Rluc cell culture system. Cells were cultured and infected as in Panel A. Increasing concentrations of the nucleoside analogue were added and followed by two days of incubation (see Materials and Methods). The levels of Renilla luciferase in cell lysates were determined as in Panel A. Cell cultures were performed in triplicate in 96-well plates, the experiment was done twice and results are reported in relative light units (RLU) as mean ± standard deviation (n=6).