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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Antiviral Res. 2010 Nov 12;89(1):54–63. doi: 10.1016/j.antiviral.2010.11.004

Figure 6. Measuring antiviral activity of benzimidazole compounds that bind the IRES of HCV RNA with JFH-ΔV3-Rluc infected cells.

Figure 6

At three hours post-infection with the JFH-ΔV3-Rluc virus (moi=0.1) Huh 7.5 cell cultures had increasing concentrations of compounds added followed by two days of incubation. Renilla luciferase activity in cell lysates was determined as in Figures 2–5. Incubations were performed in triplicate in 96-well plates, experiments were done twice, data are reported as the mean ± standard deviation (n=6) of relative light units (RLU) and used to determine the IC50 for each compound. Panel A, B and C show the results for three different benzimidazole compounds (Isis-22, 13 and 04, see Materials and Methods). Panel D shows the structure of Isis-11 which is referred to in the Discussion regarding its high-resolution NMR structure in complex with domain IIa of the HCV IRES RNA (Paulsen, et al., 2010; Seth et al., 2005).