Skip to main content
. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Antiviral Res. 2010 Nov 12;89(1):54–63. doi: 10.1016/j.antiviral.2010.11.004

Figure 7. Measuring antiviral activity of benzimidazole compounds by immunoblotting of the NS3 protein produced by HCV JFH-wt.

Figure 7

Huh 7.5 cells were infected with the JFH-wt virus, cultured, and incubated in 24-well plates with inhibitors as in Figure 6 except that cells were cultured for a total of three days to assure that the NS3 protein levels could be detected. NS3 protein levels detected by immunoblotting were used to estimate the IC50 (see Materials and Methods). The level of NS3 was normalized to the level of β-actin and the level of NS3 protein relative to controls (no inhibitor, 100% level of NS3) was plotted against inhibitor concentrations. Cell cultures were done in three independent experiments, representative immunoblots are shown for each compound (Panel A, B and C) and the data are presented as mean ± standard deviation (n=3) of the NS3 levels normalized to β-actin (Panel D).