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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Neurosci Lett. 2010 Dec 2;489(1):43–47. doi: 10.1016/j.neulet.2010.11.063

Fig. 3.

Fig. 3

Effect of chronic administration of morphine on the expression of TNF-α, IFN-γ and IL-6 in wild type mice and TLR2 KO mice. 9–10-week-old TLR2 KO male mice (n = 5) and age-matched wild type male mice (n = 6) were implanted with a 25 mg morphine pellet or a placebo pellet subcutaneously under isoflurane anesthesia as described under materials and methods. At 72 h after pellet implantation, the NAcs were harvested and total RNA was isolated. The expression of TNF-α (A) and IL-6 (B) was determined by real time quantitative RT-PCR. The level of gene expression is presented as relative mRNA expression units. Means and SEs were calculated from 5 mice per group. ** p < 0.01 compared with indicated groups.