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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Immunol Methods. 2010 Nov 13;364(1-2):50–64. doi: 10.1016/j.jim.2010.11.001

Figure 4.

Figure 4

MALDI-TOF analysis of eluted peptides using internal stable isotope labeled standards to control for variations in matrix crystallization. After capture of peptide from solution (PBS) by each RabMAb, bound peptides were eluted in 25 μL of 5% acetic acid. Stable isotope labeled peptide (500 femtomoles) was added to each eluate and after Zip-Tipping, the light and heavy peptides were spotted onto MALDI plates. All samples were ionized using a fixed laser intensity of 3400 at 500 shots/spectrum.