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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Immunol Methods. 2010 Nov 13;364(1-2):50–64. doi: 10.1016/j.jim.2010.11.001

Figure 6.

Figure 6

MiSCREEN Multiplexing. Panel A: The MALDI-TOF MS spectrum of a mixture of 5 peptides (1picomole each) that was directly spotted onto a MALDI plate. Peptide CPTAC-28d was added as a specificity control. Panel B: Forty microliters of each of four hybridoma supernatants containing RabMAbs specific for peptides CPTAC-14d, 36d, 38b and 39d were pooled and MiSCREEN was performed using this mixture. The spectrum shows that all four specific peptides were captured. Notably, the negative control peptide CPTAC-28d was not bound. Panel C. An expanded view of the spectrum shown in Panel B shows that that the control peptide was not present, even at background levels. This spectrum is representative of three independent experiments each performed with three technical replicates. Similar results were obtained in three independent experiments performed using a different peptide mixture as described in Materials and Methods (data not shown).