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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Immunol Methods. 2010 Nov 13;364(1-2):50–64. doi: 10.1016/j.jim.2010.11.001

Table 1.

Protein targets and their surrogate (proteotypic) tryptic peptides

Protein Name Surrogate Peptide ID AA Sequence Mass (Daltons)
Ferritin Light Chain CPTAC-14b KPAEDEWGK 1059.14
Ferritin Light Chain CPTAC-14d LGGPEAGLGEYLFER 1607.78
Alpha-fetoprotein precursor CPTAC-23a GYQELLEK 979.10
Alpha-fetoprotein precursor CPTAC-23c YIQESQALAK 1150.30
Receptor tyrosine-protein kinase CPTAC-36c NNQLALTLIDTNR 1485.80
Receptor tyrosine-protein kinase CPTAC-36d AVTSANIQEFAGC*K1 1495.72
Mucin-16 (CA-125) CPTAC-38b ELGPYTLDR 1063.54
Mucin-16 (CA-125) CPTAC-38c VLQGLLGPIFK 1184.74
Thyroglobulin precursor CPTAC-39c FSPDDSAGASALLR 1406.69
Thyroglobulin precursor CPTAC-39d VIFDANAPVAVR 1271.71
WAP four-disulfide core domain protein CPTAC-43c C*C*SAGC*ATFC*SLPNDK1 1847.72
LPS Binding Protein PPI-1b ITLPDFTGDLR 1247.41
LPS Binding Protein PPI-1c LAEGFPLPLLK 1197.48
Protein C Inhibitor (PCI; also known as PAI-3) PPI-4d EDQYHYLLDR 1351.44
Transferrin Receptor PPI-6d GFVEPDHYVVVGAQR 1672.86
Angiotensin-I2 Ag-I DRVYIHPFHL 1296.49
1

The asterisk (*) denotes carbamidomethyl cysteine.

2

Angiotensin-I was chosen as a positive control peptide since it ionizes extremely well and is often used as a standard in MALDI-TOF mass spectrometry.