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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Immunol Methods. 2010 Nov 13;364(1-2):50–64. doi: 10.1016/j.jim.2010.11.001

Table 3.

Detection of peptides in plasma digest1

Protein Target Peptide ID Plasma concentratio n according to literature (ng/mL)2 RabMAb ID RabMAb half- off time (min)3 Peptide detection limit (fmol)4 Peptide enriched from plasma5
Protein C Inhibitor (PCI) PPI-4d 5300 58–4 ND <10 Yes
LPS Binding Protein PPI-lc 5000 109–4 ND <10 Yes
Transferrin Receptor PPI-6d 3200 79–9 ND <10 Yes
Ferritin light chain CPTAC-14d 50 10–58 51.3 10–50 No
Receptor tyrosine-protein kinase CPTAC-36d 11.2 32–7 73.1 <10 Yes*
Mucin-16 (CA-125) CPTAC-38b 7.5 34–102 426.3 <10 Yes*
Thyroglobulin precursor CPTAC-39c 1 35–31 473.5 <10 Yes*
WAP four-disulfide core domain protein CPTAC-43c 1 38–12 194.8 <10 No
*

No accurate MS/MS analysis due to low intensity signals on MALDI-TOF MS.

1

Trypsin digested human plasma

2

Values taken from published literature

3

Determined by SPR (See also Table 2)

4

Varying levels of heavy (Stable Isotope Standard) peptide were spotted directly onto the MALDI plate.

5

Enrichment of “natural” tryptic peptide from 10 microliters of plasma digest. One microgram of the relevant RabMAb was used to enrich the peptides.