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. Author manuscript; available in PMC: 2012 Jan 25.
Published in final edited form as: Brain Res. 2010 Dec 16;1370:16–33. doi: 10.1016/j.brainres.2010.11.037

Figure 12. Cellular localization of NFs in the spinal cord.

Figure 12

Upper panel: In situ hybridization in spinal cord wholemounts showed a high level of NF95/180 mRNA expression along the lateral gray matter. L-NFL was expressed relatively evenly and weakly along the entire spinal cord. Several neuron types can be identified by their morphology and location, including: dorsal cells (DC); subependymal cells (SPC) surrounding the central canal (CC), and edge cells (EC). Note: NF132 labeled only large and medium sized cells. Lower panel: In situ hybridization in transverse sections from a 12 cm long larva (approximately 3–4 yrs old). All neurons are localized in the gray matter except for ECs, which sit along the edge of the spinal cord. MN: possible motor neuron, based on size, location and the ventrally projecting axon hillock, which is faintly labeled.