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. Author manuscript; available in PMC: 2012 Jan 25.
Published in final edited form as: Brain Res. 2010 Dec 16;1370:16–33. doi: 10.1016/j.brainres.2010.11.037

Figure 2. Recombinant L-NFL was not detected by LCM40 in Western blots.

Figure 2

The c-Myc-tagged L-NFL recombinant was expressed in SW13cl.2Vim− cells. Lysates of the cells with [SW13cl(+)] or without [SW13cl(−)] expression vector were separated on SDS-PAGE with cytoskeletal proteins prepared from lamprey spinal cord (S.C.), and transferred to nitrocellulose membrane. The membranes were then incubated with anti-Myc (Left) or LCM40 (Right) antibody. A band (~ 64 kDa) was labeled by anti-Myc but not by LCM40.