Four full-length lamprey NF cDNAs are drawn as rods in proportion to their length. The number on the right end represents the nucleotide number. The numbers below the rod represent the numbers of the nucleotides at the beginning of (from left to right) the head, rod, sidearm, and stop codon, respectively. The black line above each rod indicates the region of the individual probe used for Northern blot and in situ hybridizations. Numbers above NF180 and NF95 represent the last nucleotide of the previous fragment. Top 2 rods: NF180 was divided into 3 fragments: I, II, and III. NF95 shared two DNA fragments (I and III) with NF180 but lacked fragment II. Nucleotide sequences between fragments I & II or around fragment II and III in NF180, or the sequences between fragment I & III in NF95, were given below each rod. For fragment I: identity between NF180 and NF95 was 99.0%. For fragment III identity was 99.7%. A: Three gaps in NF95 as compared with NF180 in the fragment I region (ACC106–108, A1650, and CG1658–1659). B: Alignment analysis of L-NFL and an 843 bp sequence of a GenBank DNA fragment (Gb-Fr, TI number: 1229593736) obtained by MegaBlast search of the NCBI trace archive (Petromyzon marinus-WGS).