Table 1. Comparisons of amino acid sequence identities between newly cloned lamprey NFs and previously identified NFs within different regions of the protein.

A. The deduced amino acid sequence of lamprey NF-L was compared to the NF-Ls of other species. Sequences were aligned first to identify the tripartite regions (Head-Rod-Sidearm). For each individual region, sequences were aligned and the identities were calculated using the tool “sequence identity matrix” in BioEdit. H-NFL: Human NF-L (BC039237); R-NFL: Rat NF-L (NM_031783); M-NFL: Mouse NF-L (DQ201635); C-NFL: Chicken NF-L (XM_417679); X-NFL: Xenopus NF-L (M86654); X-nIF: Xenopus nIF (M86653); Z-NFL: Zebrafish NF-L (BC114232); L-NFL: Lamprey NF-L (DQ869188). B & C: The deduced amino acid sequences of lamprey NF132 (B) or NF95 (C) proteins were compared to NF-Ms of other species. H-NFM: human NF-M (NM_005382); R-NFM: Rat NF-M (NM_017029), M-NFM: Mouse NF-M (NM_008691); C-NFM: Chicken NF-M (NM_001101730); NF180: Lamprey NF180 (U19361); X-NFM1: Xenopus NF-M1(BC074454); X-NFM2: Xenopus NF-M2 (BC078128); Z-NFM: Zebrafish NF-M (BC133105); NF132: Lamprey NF132 (DQ398933); NF95: Lamprey NF95 (DQ398934).

A. L-NFL vs. other NF-Ls	Percentage Sequence Identity (%)
	Head	Rod	Tail	Overall
H-NFL	35	63	9.0	44
R-NFL	35	63	9.8	45
M-NFL	36	63	9.7	45
C-NFL	34	64	8.3	43
X-NFL	30	59	10	41
X-nIF	27	54	11	41
Z-NFL	25	54	7.5	34

B. NF132 vs. other NF-Ms	Percentage Sequence Identity (%)
	Head	Rod	Tail	Overall
H-NFM	36	64	13	32
R-NFM	36	64	13	35
M-MFM	37	64	14	35
C-NFM	34	64	15	33
NF180	94	98	30	55
NF95	96	99	39	72
X-NFM1	28	62	14	33
X-NFM2	34	60	16	33
Z-NFM	22	53	13	31

C. NF95 vs. other NF-Ms	Percentage Sequence Identity (%)
	Head	Rod	Tail	Overall
H-NFM	38	64	11	31
R-NFM	37	64	12	33
M-MFM	38	64	12	33
C-NFM	35	64	13	32
NF180	98	98	28	54
X-NFM1	29	62	8.7	30
X-NFM2	35	60	10	31
Z-NFM	23	53	9.6	29