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. 2010 Dec 16;48(4):291–295. doi: 10.3347/kjp.2010.48.4.291

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© 2010, Korean Society for Parasitology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Construction of pcDNA3 HA HGF vector and HGF-expression. HGF was inserted into pcDNA3 HA vector using BamHI and XhoI restriction enzyme sites. DNA fragments were observed for HGF cloning by restriction enzymes digestion (A) Lane 1, HindIII; lane 2, HindIII + XbaI. (B) Shows the expression of 25-kDa-sized HGF. The diagram of pcDNA3 HA HGF vector of about 7.4 kb which contains restriction enzyme sites is provided as supplementary data.