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PAC1-R gene expression in the 24-hpf (a–c) and 48-hpf (b–d) zebrafish embryos. Whole-mount in situ hybridization by using a DIG-labeled PAC1a-R antisense probe (a, b) or a PAC1b-R antisense probe (c, d). Diencephalon D, hypothalamus HT, midbrain–hindbrain boundary MHB, rhombencephalon R, telencephalon T, thalamus TH, and tectum TC
