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. 2010 Apr 16;20(1):167–175. doi: 10.1007/s11248-010-9390-9

Fig. 5.

Fig. 5

Germline transmission testing by crossing the chimeras with CD1 females. a All F1 offsprings showed black or agouti color but no white color, indicating thatgermline transmission from DsRed iPS cells. b These mice were confirmed with DsRed transgene by PCR genotyping. Lane 1 no DNA (negative control), lane 2 non-transgenic WT and lane 3 DsRed transgenic (positive control) showing 300 bp PCR amplicon