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. 2001 Feb 20;98(5):2610–2615. doi: 10.1073/pnas.041609298

Figure 3.

Figure 3

Ligands for RXR and LXR induce macrophage ABC-1 expression and cholesterol efflux. (A) ABC-1 mRNA levels in RAW 264.7 cells treated for 24 h with either a ligand for LXRα [22(R)-OH-Cholesterol; 10 μM] or a rexinoid (LG101305; 1 μM). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control probe. Relative fold induction of ABC-1 mRNA after normalization to the GAPDH expression is indicated under the blots. (B) ApoA-I-mediated cholesterol efflux in peritoneal macrophages obtained from control mice. Macrophages were treated with the agonists as specified under A. In addition to these treatments, macrophages were also challenged with a PPARγ agonist (BRL 49,653; 10 μM). (C) ApoA-I-mediated cholesterol efflux in peritoneal macrophages from LXRα and β double −/− mice.