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Reduction in Bcl-2 levels after treatment over time with DRE. A375 cells were treated with 2.5 mg/mL DRE for 15 and 45 minutes. Equal amounts of protein (20 μg) were separate using SDS-PAGE. Western blotting with anti-Bcl-2 antibody assessed Bcl-2 levels. Equal protein loading was confirmed by β-actin immunoblotting.
