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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Dev Biol. 2010 Nov 1;349(2):238–249. doi: 10.1016/j.ydbio.2010.10.032

Figure 1.

Figure 1

To remove presumptive cardiac neural crest at stage 7 in ovo, bilateral cuts were made with a glass needle to remove the neural folds of chick embryos having 1 or 2 somites. Incisions extended one-somite’s length rostral and caudal to the first formed somite (presumptive somite 2). The medial edge of the incision ran along the middle of the somites without damaging the underlying somitic tissue. The lateral edge of the cut was just beyond the border of the neural fold and non-neural ectoderm. The ablated tissue was removed and eggs were sealed and re-incubated until the desired stage.