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Germline transmission of the shRNA-PTEN transgene in F1 progeny
| ES clone | Hairpin | No. of F1 chimera | Germline transmission | Trangenic line |
| A | sh2 | 2 | Yes | PtenshRNA4 |
| B | sh2 | 5 | Yes | PtenshRNA5 |
| C | sh3 | 4 | Yes | PtenshRNA21 |
| D | sh3 | 2 | Sterile | none |
| E | sh3 | 1 | Sterile | none |
A summary of chimera production and subsequential germline transmission by selected shRNA-Pten ES clones. ES clones were screened for at least 50% inhibition of Pten expression level by western Blot.
