Figure 5.

(a) Myocyte area as determined by wheat germ agglutinin and DAPI costaining in control and doxorubicin-treated hearts. (b) and (c) Representative wheat germ agglutinin and DAPI costaining in control and doxorubicin-treated hearts delineating myocyte cell membranes (green) and nuclei (blue). In control animals, myocytes are of a uniform size and shape, and interstitial space is clearly visible as dark, variegated bands through the myocardium. In doxorubicin-treated animals, myocytes are hypertrophic and nonuniform in shape. 20x, white bar = 100 μm, *P < 0.05 versus control. LV, left ventricle; RV, right ventricle; n = 4/group.