Table 1.
Surface marker | Protein name | Indicator for HSC* |
---|---|---|
≥95% of the cell population must be positive for | ||
CD73 | ecto-5′-nucleotidase (SH3 and SH4) | Non-specific |
CD90 | thy-1 | Non-specific |
CD105 | endoglein (SH2) | Non-specific |
≤98% of the cell population must be negative for | ||
CD34 | Unknown antigen | Primitive hematopoietic progenitors and endothelial cells |
CD45 | Protein tyrosine phosphatase receptor type C | Pan-leukocyte marker |
CD14 or CD11b‡ | Unknown antigen or integrin α M | Monocytes and macrophages |
CD79α or CD19‡ | Immunoglobulin-associated α or unknown antigen | B-cells |
HLA-DR§ | MHC class II (heterodimer) | Immunologically simulated cells |
HSC: Hematopoietic stem cell.
Either marker is sufficient for the given requirement.
Cells positive for HLA-DR may still be considered MSCs, but this marker indicates that they have been stimulated (e.g., by IFN-γ), which should be indicated when describing the cell type (i.e., as ‘stimulated’ MSCs).
The proteins associated with CD34, CD19 and CD19 are currently unknown.