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Time course of whole-body fluorescence imaging of MDA-MB-435-GFP human breast cancer angiogenesis in orthotopic primary tumor. The GFP-expressing human tumor was transplanted by SOI in the fat pad of nude mice and whole-body imaged as described in Materials and Methods. (A) Week 1. (B) Week 4. (C) Week 8. (D) Week 20. (E) Quantitative graph of microvessel density as a function of time.
