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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: Circ Res. 2010 Aug 19;107(8):959–966. doi: 10.1161/CIRCRESAHA.110.219592

Figure 4. Analysis of VE-cadherin luciferase promoter reporter constructs.

Figure 4

A, Human wild-type VE-cadherin promoter and truncated promoter constructs driving luciferase reporter gene. B, Time-line of transfection and luciferase assay. C, Indicated constructs were transiently transfected into ECs together with a tracer amount of β-galactosidase. Fold-luciferase activity is shown as mean ± s.e.m. *p < 0.05 vs control (untreated) group; **p <0.01 vs control (without reporter construct) calculated from three independent experiments, each carried out in triplicate. D and E, Transfection efficiency with or without addition of WNT3A was determined by staining with X-gal.