. Author manuscript; available in PMC: 2011 Nov 19.

Published in final edited form as: J Mol Biol. 2010 Oct 1;404(1):34–44. doi: 10.1016/j.jmb.2010.09.015

Table 2.

Steady-state kinetic parameters for one-base incorporation opposite an AP site and G by human pols η, ι, κ, and REV1

Polymerase	Template	dNTP	K_m (µM)	k_cat (s⁻¹)	k_cat/K_m (mM⁻¹ s⁻¹)	dNTP selectivity ratio^a	Relative efficiency^b
η	AP site	A	40 ± 6	0.12 ± 0.004	3.0	0.95	0.065
		T	290 ± 50	0.92 ± 0.05	3.2	1	0.070
		G	8.5 ± 1.0	0.005 ± 0.0001	0.59	0.19	0.013
		C	210 ± 20	0.14 ± 0.01	0.67	0.21	0.015
	G	C	2.6 ± 0.1	0.12 ± 0.005	46		1

ι	AP site	A	210 ± 40	0.54 ± 0.04	2.6	0.45	1.4
		T	130 ± 20	0.74 ± 0.02	5.7	1	3.0
		G	120 ± 10	0.47 ± 0.01	3.9	0.69	2.1
		C	570 ± 140	0.77 ± 0.05	1.4	0.24	0.74
	G	C	300 ± 30	0.57 ± 0.02	1.9		1

κ	AP site	A	1600 ± 200	0.077 ± 0.005	0.048	0.77	0.00065
		T	2300 ± 700	0.017 ± 0.002	0.0074	0.12	0.00010
		G	400 ± 70	0.0032 ± 0.0002	0.008	0.13	0.00011
		C	780 ± 220	0.049 ± 0.005	0.063	1	0.00085
	G	C	3.8 ± 0.5	0.28 ± 0.01	74		1

REV1	AP site	A	140 ± 50	0.000025 ± 0.000002	0.00018	0.0031	0.00019
		T	190 ± 30	0.000072 ± 0.000003	0.00038	0.0067	0.00040
		G	190 ± 50	0.000031 ± 0.000003	0.00016	0.0029	0.00017
		C	210 ± 30	0.012 ± 0.001	0.057	1	0.061
	G	C	12.8 ± 50	0.012 ± 0.0003	0.94		1

dNTP selectivity ratio, calculated by dividing k_cat/K_m for each dNTP incorporation by the highest k_cat/K_m for dNTP incorporation opposite AP site.

Relative efficiency, calculated by dividing k_cat/K_m for each dNTP incorporation opposite AP site by k_cat/K_m for dCTP incorporation opposite G.