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. Author manuscript; available in PMC: 2011 Nov 19.
Published in final edited form as: J Mol Biol. 2010 Oct 1;404(1):34–44. doi: 10.1016/j.jmb.2010.09.015

Table 3.

Steady-state kinetic parameters for one-base incorporation opposite an AP site and G by human pols α and δ/PCNA

Polymerase Template dNTP Km (µM) kcat (s−1) kcat/Km
(mM−1 s−1)		 dNTP
selectivity ratioa 		Relative
efficiencyb
α AP site A 570 ± 100 0.0083 ± 0.0001 0.015 1 0.0010
T 250 ± 60 0.00046 ± 0.00003 0.0018 0.12 0.00012
G 550 ± 120 0.00024 ± 0.00002 0.0004 0.027 0.00003
C 980 ± 50 0.00047 ± 0.000001 0.0005 0.033 0.00003
G C 0.42 ± 0.09 0.0064 ± 0.0003 15 1
δ/PCNA AP site A 25 ± 6 0.0067 ± 0.0004 0.27 1 0.012
T 62 ± 16 0.0060 ± 0.0004 0.097 0.36 0.0044
G 110 ± 20 0.010 ± 0.001 0.091 0.34 0.0041
C 880 ± 160 0.0069 ± 0.0006 0.0078 0.029 0.0004
G C 0.27 ± 0.05 0.0059 ± 0.0002 22 1
a

dNTP selectivity ratio, calculated by dividing kcat/Km for each dNTP incorporation by the highest kcat/Km for dNTP incorporation opposite AP site.

b

Relative efficiency, calculated by dividing kcat/Km for each dNTP incorporation opposite AP site by kcat/Km for dCTP incorporation opposite G.