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. Author manuscript; available in PMC: 2011 Nov 19.
Published in final edited form as: J Mol Biol. 2010 Oct 1;404(1):34–44. doi: 10.1016/j.jmb.2010.09.015

Table 4.

Steady-state kinetic parameters for next base extension from an AP site:A (or T, G, C) pair and G:C pair template:primer termini by human pols η and κ

Polyme
rase		 Base-pair at 3’
primer termini
(template:primer)		 Extension with dGTP (the correct nucleotide against next template C)
Km (µM) kcat (s−1) kcat/Km
(mM−1 s−1)		 Relative base-pair
selectivity ratioa 		Relative
extension
efficiencyb
η AP:A 4.7 ± 0.8 0.011 ± 0.001 2.3 1 0.066
AP:T 11 ± 3 0.0015 ± 0.0001 0.14 0.06 0.0040
AP:G 11 ± 2 0.0037 ± 0.0001 0.34 0.15 0.0097
AP:C 6.4 ± 1.2 0.0021 ± 0.0001 0.33 0.14 0.0040
G:C 0.43 ± 0.12 0.015 ±0.001 35 1
κ AP:A 980 ± 40 0.0044 ± 0.0001 0.0045 0.58 0.00035
AP:T 120 ± 30 0.00092 ± 0.00006 0.0077 1 0.00059
AP:G 400 ± 100 0.00066 ± 0.00005 0.0017 0.22 0.00013
AP:C 80 ± 20 0.00059 ±0.00004 0.0074 0.96 0.00057
G:C 5.3 ± 1.1 0.070 ± 0.004 13 1
a

Relative base-pair selectivity ratio, calculated by dividing kcat/Km for each dGTP incorporation by the highest kcat/Km for dGTP incorporation opposite the next template C from the AP site:N (N=A, T, G, or C) pair.

b

Relative extension efficiency, calculated by dividing kcat/Km for each dGTP incorporation opposite the next template C from the AP site:N pair by kcat/Km for dGTP incorporation opposite the next template C from the normal G:C pair.