. Author manuscript; available in PMC: 2011 Nov 19.

Published in final edited form as: J Mol Biol. 2010 Oct 1;404(1):34–44. doi: 10.1016/j.jmb.2010.09.015

Table 5.

Steady-state kinetic parameters for next base extension from T:A and AP site:A template:primer termini by human pols α and δ/PCNA

Polyme rase	Base-pair at 3’ primer termini (template:primer)	Extension with dGTP (the next correct nucleotide against template C)
Polyme rase	Base-pair at 3’ primer termini (template:primer)	K_m (µM)	k_cat (s⁻¹)	k_cat/K_m (mM⁻¹ s⁻¹)	Relative extension efficiency^a
α	T:A	0.78 ± 0.10	0.015 ± 0.001	19	1
α	AP:A	500 ± 50	0.00017 ± 0.00001	0.00034	0.000018

δ/ PCNA	T:A	0.18 ± 0.0004	0.0074 ± 0.0004	41	1
δ/ PCNA	AP:A	280 ± 80	0.0010 ± 0.0001	0.28	0.0068

Relative extension efficiency, calculated by dividing k_cat/K_m for each dGTP incorporation opposite the next template C from the AP site:A pair by k_cat/K_m for dGTP incorporation opposite the next template C from the normal T:A pair.