Table 1.
The Forward and Reverse Primers for the PCR-Based Mutagenesis Experiments
| Mutanta | Primerb |
|---|---|
| SaE22A | 5′-GGTGCTTTATCAGCTGCAAATGAAATAGGGCAAATTTTC-3′ |
| 5′-GAAAATTTGCCCTATTTCATTTGCAGCTGATAAAGCACC-3 | |
| SaE74A | 5′-GCCGTTAATTTACTTGCGCATCTAGCTGAACGTATTGC-3′ |
| 5′-GCAATACGTTCAGCTAGATGCGCAAGTAAATTAACGGC-3′ | |
| SaK100A | 5′-GAAACGAAAGTGAGAATCACTGCAGAAAACCCACCGATTCCG-3′ |
| 5′-CGGAATCGGTGGGTTTTCTGCAGTGATTCTCACTTTCGTTTC-3′ | |
| SaK100Q | 5′-CGAAAGTGAGAATCACTCAAGAAAACCCACCGATTCC-3′ |
| 5′-GGAATCGGTGGGTTTTCTTGAGTGATTCTCACTTTC G-3′ | |
| EcE21A | 5′-GTGTTTACGACTGGGCACAGACCATCGAACAG-3′ |
| 5′-CTGTTCGATGGTCTGTGCCCAGTCGTAAACAC-3′ | |
| EcE73A | 5′-GCGCTGGTGGCACGCGTGGCTG-3′ |
| 5′-CAGCCACGCGTGCCACCAGCGC-3′ | |
| EcK98A | 5′-CGTATCAAACTCAGCGCGCCAGGCGCAGTGG-3′ |
| 5′-CCACTGCGCCTGGCGCGCTGAGTTTGATACG-3′ | |
a
SaE22A, SaE74A, and SaK100A are mutants of SaDHNA in which E22, E74, and K100 are replaced by alanine respectively. SaK100Q is a SaDHNA mutant with K100 replaced by glutamine. EcE21A, EcE73A, and EcK98A are mutants of EcDHNA in which E21, E73, and K98 are replaced by alanine respectively.
b
The forward primers are listed first with the mutations underlined. The mutations in the reverse primers are not indicated.