Table 3.

Binding Constants of EcDHNA and Site-Directed Mutants

		EcDHNA	EcE21A	EcE73A	EcK98A
NP	Kd (μM)	0.77±0.06	1.7±0.01	260±40	11±0.3
	k1 (M−1s−1)	(3.2±0.2)×105	(4.6±0.2)×105	n.d.a	(6.6±0.1)×105
	k−1 (s−1)	0.29±0.03	0.82±0.02	n.d.	7.2±0.3
MP	Kd (μM)	2.6±0.06	0.80±0.01	420±20	9.4±0.09
	k1 (M−1s−1)	(2.6±0.1)×105	(4.3±0.2)×105	n.d.	(9.1±0.4)×105
	k−1 (s−1)	0.58±0.03	0.47±0.02	n.d.	9.1±0.6
HPO	Kd (μM)	0.10±0.007	4.2±0.01	560±20	23±2
	k1 (M−1s−1)	(5.5±0.4)×105	(1.4±0.1)×106	n.d.	(1.2±0.1)×106
	k−1 (s−1)	0.062±0.006	6.3±0.2	n.d.	27±1

^an.d.: not determined.