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Schematic depiction of the cellular microarray assay in which soluble peptide-loaded HLA A2-Ig dimers are incubated with T cells in solution. The antigen-specific sub-population of cells are identified by the surface-bound pHLA A2-Ig dimers and subsequently captured on the microarray by binding to printed anti-Igλ antibodies that recognize the immunoglobulin (Ig) portion of the dimer.
