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. 2010 Nov 26;29(24):4118–4131. doi: 10.1038/emboj.2010.292

Figure 3.

Figure 3

Enhanced ROS accumulation in Foxo3−/− primitive myeloid progenitors. Representative FACS profile of bone marrow Lin− IL7Rα− Sca-1− c-Kit+ that contain 98% of all myeloid progenitor cells (Akashi et al, 2000) (left panel). Frequency of c-Kit+ Sca1− cells within Lin− IL7Rα− cells is shown. Endogenous ROS concentrations were measured in freshly isolated Lin− IL7Rα− Sca-1− c-Kit+ cells (right panel) from wild-type or Foxo3−/− mice treated daily in vivo with NAC (100 mg/kg) or PBS for 15 days; fold change in mean fluorescence intensity (MFI) of ROS in gated subpopulations (ROS-hi using - - - - - - - gate), as compared with control wild-type cells treated with PBS is shown as mean±s.e.m., n=3; Student's t-test. One of two independent experiments is shown.