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. 2010 Nov 26;29(24):4118–4131. doi: 10.1038/emboj.2010.292

Figure 7.

Figure 7

Deregulated expression of modulators of cytokine signalling in Foxo3−/− hematopoietic progenitor cells. (A) QRT–PCR expression analysis of cytokine receptor signalling regulatory genes in lineage-negative wild-type and Foxo3−/− cells. Quantification of target genes is relative to β-actin. Results are mean±s.e.m. of duplicate analysis of at least three cDNAs, each generated from a pool of two to three wild-type or Foxo3−/− mice; Student's t-test. (B) Western blot analysis of endogenous Lnk protein in total bone marrow cells isolated from two wild-type and two Foxo3−/− mice; representative immunoblot of three independent experiments is shown. (C) QRT–PCR analysis of Foxo3 (upper panel) and Lnk (lower panel) expression in mononuclear cells derived from 5-FU-treated wild-type and Foxo3−/− mice, transduced with MSCV-IRES-GFP (MIG) vector control or MIG-Foxo3; results are mean±s.e.m. of three independent experiments.