Skip to main content
. Author manuscript; available in PMC: 2011 Oct 29.
Published in final edited form as: Chem Biol. 2010 Oct 29;17(10):1101–1110. doi: 10.1016/j.chembiol.2010.07.017

Figure 4. TarK and TarL react sequentially on the TarB product (4).

Figure 4

(A) PAGE analysis of a tandem TarKBs/TarLBs reaction using either heat-treated (−) or active (+) enzymes incubated with CDP-ribitol and 4b, 5b, or 6b. The polymeric material in the middle panel is due to reaction with contaminating 4b. Polymer product only forms when 4b is used as the starting material (see text).

(B) PAGE analysis of TarLBs heat-treated (−) or active (+) enzyme incubated with 4b or 7b and CDP-ribitol (2). TarLBs reacts only with 7b. See also Figure S4.

(C) Reactions carried out by TarKBs and TarLBs with CDP-ribitol (2) and substrates 4 and 7 respectively.