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. Author manuscript; available in PMC: 2011 Oct 29.
Published in final edited form as: Chem Biol. 2010 Oct 29;17(10):1101–1110. doi: 10.1016/j.chembiol.2010.07.017

Figure 5. TarKBs and TarLBs make polyRboP-WTAs in vivo in the absence of TarFBs.

Figure 5

(A) Growth curves of B. subtilis W23 wildtype (WT) or ΔtarF strains.

(B) Photograph of bacterial colonies of B. subtilis W23 wildtype (WT) or ΔtarF strains. (C,D) WTAs of the parent wildtype strain or the ΔtarF strain extracted from the B.subtilis/S. aureus hybrid strain JT215 (C) or B. subtilis W23 (D) analyzed by PAGE analysis and alcian blue/silver staining.

(E) Agarose gel analysis of PCR amplified fragments from B. subtilis W23 cDNA or genomic DNA (positive control). (+) and (−) indicate the presence or absence of reverse transcriptase during cDNA synthesis. The sizes of the amplified fragments are 170 bp (tarK), 159 bp (tarF), and 68 bp (16S rRNA) (internal control).