Fig. 12.

Pon3 mRNA expression in ApoE −/− adult mouse heart. (a) Emulsion autoradiography detection of Pon3 mRNA seen as bright labeling under darkfield illumination. Labeling occurs in several tissues including the aortic root, vein, cardiac muscle, and connective tissue around the heart. Labeling also occurs in the atherosclerotic lesion area (arrow). (b) Cresyl violet staining of the same section shown in (a) seen under brightfield illumination. (c) Control hybridization in an adjacent section comparable to (a). (d) Cresyl violet staining of the same section shown in (c) seen under brightfield illumination. Abbreviations: AR – aortic root; Bl – blood; CM – cardiac muscle; CT – connective tissue; Ve – vein; (as) – antisense; (s) – sense