Table 1.
Effect of TSA on CD154 surface expression, IL10 production, and IFN-γ secretion in SLE and control T cells
| 24-h culture | CD154+ T cells
(%) Mean ± SEM
|
IL-10 (pg/ml) Mean ±
SEM
|
IFN-γ (pg/ml) Mean ± SEM
|
|||
|---|---|---|---|---|---|---|
| Normal | SLE | Normal | SLE | Normal | SLE | |
| Medium | 4.1 ± 1.4 | 5.0 ± 1.2 | 0 | 242.4 ± 144.2 | 0 | 0 |
| TSA (1000 ng/ml) | 2.5 ± 0.8 | 2.1 ± 0.7 | 0 | 0 | 70.8 ± 27.4 | 0 |
| PMA (20 ng/ml) + IO (0.5 μM) | 26.7 ± 4.0 | 24 ± 3.2 | 0 | 65.9 ± 49.3 | 2862.2 ± 1258.8 | 23.8 ± 13.9 |
| TSA (1000 ng/ml) + PMA (20 ng/ml) + IO (0.5 μM) | 5.6 ± 1.6 | 12.3 ± 3.2 | 0 | 0 | 2384.0 ± 970.9 | 291.4 ± 143.4 |
| P value | ||||||
| Medium, TSA | P = N.S. | P = 0.029 | P = 0.008 | |||
| PMA + IO, TSA + PMA + IO | P < 0.001 | P = 0.005 | P = 0.299 | P = 0.011 | ||