Fig. 4.

Prolongation of animal survival times and elimination of preformed xenograft tumors. SVV-001 (5 × 10¹² vp/kg) was administered through a single tail vein injection 2 and 4 weeks after tumor cell transplantation (n = 10 per group). (A) Log-rank analysis of animal survival times showing significant improvement of animal survival times in mice treated with SVV-001 (P < .01). (B) Hematoxylin and eosin (H&E) staining of paraffin sections showing elimination of xenograft tumors in 8 of the 10 long-term survivors, compared with the huge ICb MB xenografts in the mock-treated control groups (arrow). (C) IHC staining with human-specific antibodies against mitochondria in the 2 ICb-1299MB (mice #1 and #2) and 6 ICb-1572MB (mice #5–10) mice in which no residual tumors were observed with H&E staining. Compared with the intense positivity (arrow) detected in the positive control section, no MT-positive cells were detected in the 8 brains, though a disturbance of the granular layer and micronodules with empty/shallow cytoplasm and condensed nuclei (circled in red) were seen. Magnification: ×40.