Fig. 3.
In vitro quantitative analysis of TRAIL expression in hAT-MSC.TRAIL cells and their therapeutic efficacy on F98 cells. TRAIL protein secreted into hAT-MSC.TRAIL medium was quantified using ELISA. hAT-MSC.TRAIL supernatant contained increased levels of TRAIL, in a cell number-dependent fashion. Approximately 5 × 103 hAT-MSC.TRAIL cells secreted 1 ηg/mL of TRAIL protein (A). The therapeutic efficacy of hAT-MSC-TRAIL cells was analyzed in coculture experiments. F98 cells cultured in the presence of hAT-MSC-TRAIL cells exhibited significant growth inhibition; however, there was no significant growth inhibition in coculture with hAT-MSC cells (B). Columns represent cell viability as a percentage of the control viability; bars, SE (P < .05, Kruskal–Wallis test with post hoc analysis).