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. 2010 Aug 17;12(12):1231–1243. doi: 10.1093/neuonc/noq101

Fig. 1.

Fig. 1.

Jagged1 ligand action on SH-SY5Y neuroblastoma cell growth. (A) Western blot analysis of NICD protein expression levels after 24 and 48 hours of Jagged1 peptide (J1) treatment. GAPDH was used as a loading control. (B) QRT-PCR analysis of HES1 mRNA expression levels after 24 and 48 hours of J1 treatment. (C) Immunofluorescence of SH-SY5Y cells grown for 48 hours in culture medium alone (CTR) or in medium added with 40 µM J1 peptide; βIII tubulin antibody was used to indentify neuroblastoma cells. (D) Cell number determination of SH-SY5Y treated with an increasing dose of J1 for 48 hours. (E) Determination of Ki-67 positive cells in SH-SY5Y neuroblastoma cells untreated or treated for 48 hours with J1 synthetic peptide. *P < .001 vs control.