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. 2001 Feb 20;98(5):2634–2639. doi: 10.1073/pnas.051619598

Figure 2.

Figure 2

In vivo and in vitro assessment of left ventricular function and carotid artery reactivity in TK+/+ and TK−/− mice. (A) Fractional shortening determined by two-dimensional echocardiography in TK+/+ (n = 25) and TK−/− (n = 20) mice of identical body weight (25.65 ± 0.52 versus 25.63 ± 0.44 g). (B–E) Dynamic cardiac parameters measured at steady state with an impedance catheter in TK+/+ (n = 7) and TK−/− (n = 6) mice. (F) Cardiac response to acute β-adrenergic stimulation in the same animals used for the determination of baseline fractional shortening. Fractional shortening (FS) was measured before and 10 min after intraperitoneal injection of dobutamine (4 μg/g of body weight). (G) Left ventricular function curve determined in work-performing hearts isolated from TK+/+ (n = 5) and TK−/− (n = 5) mice and perfused with a fixed preload pressure of 10 mmHg. (H) Effect of luminal flow rate on the outer diameter of carotid arteries isolated from TK+/+ (n = 7) and TK−/− (n = 6) mice. The initial artery diameter precontracted by phenylephrine and the final passive diameter were identical between TK+/+ and TK−/− mice (482 ± 10 versus 500 ± 12 μm) and (552 ± 6 versus 562 ± 11 μm), respectively. Diameter was also comparable between TK+/+ and TK−/− mice in arteries exposed to 10−6 M acetylcholine (536 ± 8 versus 543 ± 11 μm). Values (means ± SEM) have been compared by ANOVA.